The application of endoscopic debridement combined with metagenomic next-generation sequencing technology in primary spinal infections: a retrospective study

Feng, Xiaofei; Cheng, Jie; Jiang, Luyong; Lin, Jiayi; Ye, Zhewei; Pang, Qingjiang; Liu, Jiangtao

doi:10.1186/s13018-024-05385-5

Journal of Orthopaedic Surgery and Research

Table 3 Comparison of clinically confirmed pathogen microorganism results with metagenomic sequencing results

From: The application of endoscopic debridement combined with metagenomic next-generation sequencing technology in primary spinal infections: a retrospective study

Patient ID	Pathogenic microorganisms	Diagnostic Basis	Results of mNGS and sequence number	Antibiotic
1–11	Stapyhlococcus aureus	Bacterial culture	Stapyhlococcus aureus	Penicillin
12–14	MRSA	Bacterial culture	Stapyhlococcus aureus	Vancomycin
15–16	Streptococcus sanguis	Bacterial culture, PCR	Prohyromonas endodontalis, Treponema deticola, Streptococcus sanguis	Penicillin Metronidazole
17	Streptococcus intermedium	Bacterial culture	Streptococcus intermedium	Linezolid, Minocycline
18–19	Streptococcus constellations	Bacterial culture	Streptococcus constellations	Linezolid, Minocycline
20–34	Escherichia coil	Bacterial culture	Escherichia coil	Cephalosporin, Levofloxacin
25–27	Pseidomonas aeruginosa	Blood culture	Pseidomonas aeruginosa	Cephalosporin, Levofloxacin
28–29	Bacteroides fragilis	Blood culture	Bacteroides fragilis	Carbapenems, Metronidazole
30	Aspergillus flavus	Bacterial culture	Aspergillus flavus	Metronidazole
31–39	Mycobacterium tuberculosis	Pathology, PCR	Mycobacterium Tuberculosis complex	Rifampicin, Isoniazid, Ethambutol, Pyrazinamide
40	Unknown pathogen	Pathology	Streptococcus pseudopneumoniae	Rifampicin, Isoniazid, Ethambutol, Pyrazinamide
41	Unknown pathogen	Pathology	Escherichia coli	Vancomycin Carbapenems
42	Unknown pathogen	Pathology	Streptococcus sinensis	Cephalosporin, Levofloxacin
43	Unknown pathogen	Pathology	Staphylococcus epidermidis	Linezolid, Levofloxacin
44–46	Unknown pathogen	Pathology	Staphylococcus aureus	Linezolid, Levofloxacin
47	Unknown pathogen	Pathology	Negative	Vancomycin
48	Unknown pathogen	Pathology	Negative	Cephalosporin, Levofloxacin
49	Unknown pathogen	Pathology	Negative	Carbapenems
50–52	Spinal metastasis	Pathology	Negative	N/A
53–58	Modic changes	Pathology	Negative	N/A
59–62	Endplate fracture	Pathology	Negative	N/A

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ISSN: 1749-799X

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