Fig. 3
From: Inhibition of id-1 reduces osteosarcoma growth and metastasis through mediation of snail
Silencing Id-1 reduces Snail expression and inhibits EMT in OS cells. (A) RT-qPCR for Id-1 and Snail mRNA expression; (B) WB for Id-1 and Snail protein content; (C) Cell proliferation was assessed using the MTT assay; (D) WB for E-cadherin, N-cadherin and Vimentin protein content; (E) Cell Scratch Assay for cell migration(100×, scale = 400 μm); (F) Transwell for cell invasion(200×, scale = 200 μm). n = 3, data results were analyzed using one-way ANOVA, **p < 0.01 vs. sh-NC, ***p < 0.001 vs. sh-NC, ****p < 0.0001 vs. sh-NC