Fig. 4
From: Inhibition of id-1 reduces osteosarcoma growth and metastasis through mediation of snail
Downregulation of Id-1 can improve the growth of subcutaneous tumors and lung metastasis in OS mice. (A) RT-qPCR for Id-1 and Snail mRNA expression; (B) WB for Id-1 and Snail protein content; (C) OS cell-injected mice tibia treated to observe the changes of body weight and osteosarcoma volume, data results were analyzed using two-way ANOVA; (D) HE to observe OS tumour pathology(100×, scale = 400 μm; 400×, scale = 100 μm); (E) number of surface nodules on lung tissue; (F) HE to observe lung histopathology(40×, scale = 1000 μm; 100×, scale = 400 μm). n = 6, data results were analyzed using one-way ANOVA, *p < 0.05 vs. sh-NC, ***p < 0.001 vs. sh-NC, ****p < 0.0001 vs. sh-NC