Fig. 6

Supernatant of osteoclast after Fer-1 treatment promotes osteogenesis and angiogenesis. (A-D) Concentration of TGF-β, SDF-1, S1P and PDGF-BB in the supernatant of osteoclast of each group. (E) ALP staining of BMSCs of each group after co-culture with supernatant of osteoclast for 14 days. Scale bar = 200 μm. (F) Positive area of ALP staining. (G) Alizarin Red staining of BMSCs of each group after co-culture with supernatant of osteoclast for 14 days. Scale bar = 200 μm. (H) Positive area of Alizarin Red staining. (I) Tube formation assay of ECs of each group after co-culture with supernatant of osteoclast for 6 h. (J) Numbers of Meshes of Tube formation assay. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not statistically significant