Fig. 7

Fer-1 inhibits osteoporosis of OVX mice. (A) Reconstruction of bone trabecular of distal femurs by Micro-CT of each group. Scale bar = 200 μm. (B-F) Quantitative micro-CT analysis of bone mineral density (BMD), bone volume/total volume ratio (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp). (G) HE staining of tissue sections, followed by the quantitative analysis of Tb.N and Tb.Sp of HE staining. Scale bar = 200 μm. (H) Relative concentration of TRAP, RANKL, CTX-1 and PINP in serum of each group. (I) TRAP staining of tissue sections. Scale bar = 200 μm. (J) Quantitative analysis of osteoclast number per trabecular bone surface (N. OC/BS). Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not statistically significant