Fig. 2
From: MiR-147b-3p promotes osteogenesis by targeting NDUFA4 and PI3K/AKT pathway
Effects of miR-147b-3p overexpression and inhibition on MC3T3-E1 cell osteogenic differentiation. (A) miR-147b-3p expression was confirmed using qRT-PCR. (B)MC3T3-E1 cells were transfected with miR-147b-3p mimic(50nM) or miR-147b-3p inhibitor （100nM）and examined for cell viability by CCK-8 assay. (C, D) Representative images of ARS staining of MC3T3-E1 cells treated with miR-147b-3p mimic or miR-147b-3p inhibitor in osteogenic induction medium for 14 days, respectively. Examined for the protein levels of ALP and Runx2 using immunoblotting on day 14 of osteogenic induction (E, F). *P < 0.05, **P < 0.01 compared to mimic NC, ##P< 0.01 compared to inhibitor NC