Fig. 6
From: MiR-147b-3p promotes osteogenesis by targeting NDUFA4 and PI3K/AKT pathway
The miR-147b-3p/NDUFA4 axis regulates the osteogenic differentiation in MC3T3-E1 cells. (A)The transfection efficiency of p-NDUFA4 was detected by qRT-PCR. Subsequently, co-transfection of NDUFA4 overexpression (p-NDUFA4) and miR-147b-3p mimic was performed in MC3T3-E1 cells. Western blotting analysis (B,C) revealed expression levels of NDUFA4 protein, while cell viability was assessed using the CCK-8(D).Then, MC3T3-E1 cells were co-transfected with p-NDUFA4 and miR-147b-3p mimic and induced for osteogenic differentiation for 14 days. Alizarin red staining (E,F) allowed observation of mineralized nodule formation during osteogenesis induction.Western blotting analysis of osteogenic induction demonstrated levels of ALP and Runx2 proteins (G,H). Data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared to NC, ##P < 0.01 compared to NC