Fig. 4

Cisplatin increased LOX-1 upregulation. A LOX-1 mRNA expression ratio. This ratio was increased after cisplatin treatment but decreased after three inhibitor treatments and NAC treatment. B LOX-1 protein expression was analyzed using Western blotting. C Cisplatin-enhanced LOX-1 expression through the ERK–p38–NFkbp65-related pathway. LOX-1 expression was downregulated after three inhibitor treatments and NAC treatment. Significant differences were obtained when comparing three inhibitor treatments and NAC treatment with cisplatin treatment alone. *p < 0.05 comparison with control; and #p < 0.05: comparison with cisplatin group, respectively