Fig. 2

HUVEC-exos inhibited osteoclast differentiation in vitro. A Representative images of PKH67 (green) and DAPI (Blue) immunostaining, scale bar = 100 nm. B Representative images of TRAP staining of RAW264.7 cells treated with RANKL or RANKL + HUVEC Exos in vitro, scale bar = 100 nm. C quantiative analysis of positive TRAP staining in Fig. 2B (*P < 0.05). D Expression levels of Ctsk and Nfatc1 in RAW264.7 cells treated with RANKL or RANKL + HUVEC Exos in vitro (*P < 0.05)