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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Icariin promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by activating PI3K-AKT-UTX/EZH2 signaling in steroid-induced femoral head osteonecrosis

Fig. 5

Like icariin, overexpressed UTX or silenced EZH2 exerts the same inhibitory effect on adipogenic differentiation of BMSCs. (A) Oil-red O staining was performed to measure the influence of icariin, UTX upregulation, EZH2 depletion, or PTH on adipogenic differentiation of BMSCs in presence of DEX. (B-C) RT-qPCR was performed to assess mRNA levels of adipogenesis-related markers (PPAR-γ and C/EBPα) in the control, DEX, DEX + icariin-M, DEX + UTX, DEX + sh-EZH2, and DEX + PTH groups. (D-F) PPAR-γ and C/EBPα protein levels in BMSCs of abovementioned groups were measured by western blotting. ***p < 0.001 versus control group, ###p < 0.001 versus DEX group

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X