Fig. 6

Immunofluorescence images of ACAN of intervertebral disc tissues with different types of herniation. Blue fluorescence represents DAPI staining of nucleus, green fluorescence represents ACAN staining. In normal group, the nucleus pulposus was full and green fluorescence was aggregated; the degeneration intervertebral disc nucleus pulposus was destroyed and the fluorescence area was reduced; the central and paramedian herniation discs detected green fluorescence signals at the herniated site