Fig. 1

Identification and functional analysis of candidate genes associated with OS and PMRGs. A Sample clustering analysis of the GSE99671 dataset. B Screening of the optimal soft threshold. C Cluster dendrogram and module overview. D Correlations between merged modules and OS. E–F Volcano plot (E) and heatmap (F) showing DEGs between OS and normal tissues. G–H GO (G) and KEGG (H) enrichment analysis of the candidate genes. OS, osteosarcoma; PMRGs, polyamine metabolism-related genes; DEGs, differentially expressed genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes