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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Electroacupuncture attenuates intervertebral disc degeneration by upregulating aquaporins via the cAMP/PKA pathway

Fig. 3

AQP1and AQP3 protein expression in disc degeneration. (A) Representative microscopic photographs of immunofluorescence staining showing AQP1 and AQP3 immunoreaction-positive products. Nuclei counterstained with DAPI showed blue, with AQP1 and AQP3 positive products appearing green. Scale bar: 20 μm. The average optical density (AOD) was measured. (B,C) Bar graphs showing the AQP1 and AQP3 immunoreaction-positive products. (D) Western blot analysis of AQP1 and AQP3 protein levels. GAPDH was analyzed as a housekeeping gene. (E, F) Bar graphs showing the protein levels of AQP1 and AQP3. (G, H) Bar graphs showing the mRNA levels of AQP1 and AQP3. Data are expressed as mean ± SD; **P < 0.01 compared to the sham compressed group; ##P < 0.01 and #P < 0.05 compared to the model group

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X