Fig. 2

Effects of differentiation on gene expression and cells. (A) Differentiation treatment increased miR-654-3p levels in cells; (B) Differentiation treatment increased ALP, OCN, and Runx2 levels in cells; (C) Decreased cell proliferation capacity due to differentiation treatment; (D) Elevated apoptosis due to differentiation treatment; (E) Differentiation treatments resulted in elevated pro-apoptotic factors and decreased inhibitory apoptotic factors (*** P < 0.001 vs. control)