Fig. 5

Regulatory role of miR-654-3p and EMP1. (A) Predicted binding sites of miR-654-3p and EMP1; (B) Differentiation treatment reduced EMP1 levels in cells; (C) DLR tests confirm target recognition relationship of miR-654-3p with EMP1; (D) Effect of transfection on miR-654-3p levels; (E) Effect of transfection on EMP1 levels; (F) Effect of transfection on EMP1 protein content; (G) Both miR-654-3p and EMP1 were predominantly enriched in anti-Ago-2 (*** P < 0.001 vs. control or OI, ### P < 0.001 vs. OI + miR-inhibitor + EMP1-NC)