Fig. 5

Obacunone (OB) acts as an inhibitor of HDAC1 to limit p38MAPK signaling. A Venn diagram of the targets for OB and OA. B GO and KEGG analysis of downstream signaling pathways for OB. C The 3D views of molecular docking of OB and its target proteins (HDAC1, 2, 3, 4, and 9) using MOE software. D PPI network was made using STRING database and visualized using (E) Cytoscape3.10.0. (F) The top ten hub genes in PPI network. G The primary chondrocytes were treated with 20, 40, and 80 μM OB in the absence and presence of IL-1β. Western blot was performed to detect the protein expression of HDAC1. H A protein synthesis inhibitor (CHX) was added to incubate cells for 0~24 h, the protein stability of HDAC1 under OB treatment was analyzed. I Western blot was performed to detect the activation of p38MAPK. n = 3. Data were analyzed by two-way ANOVA followed by Bonferroni's post hoc test. ***P<0.001