Fig. 6

Proteomics analysis of the CM in control and TNFRSF11B-OE groups. (A, B) Differential protein expression between the two sample groups is depicted using a heat map and bar chart. In the heat map, blue represents downregulation, while red denotes upregulation. (C) The radar chart illustrates the relative expression levels of DEPs. The innermost circle ranks the differential proteins clockwise along the orange arrows based on p-values from smallest to largest. The second circle displays the Log2-transformed ratio, with pink indicating upregulation and light blue representing downregulation, while larger dots denote greater fold changes. The third circle indicates the average quantification level of the two groups, where spikes in the graph signify highly expressed differential proteins. (D) The figure presents the distribution of differential proteins across various subcellular structures, along with their proportional representation in each category. Pink indicates upregulated proteins, whereas blue represents downregulated proteins. (E) The GO secondary classification of DEPs. Capital letters “A” and “B” in (A–C) correspond to the control-CM and TNFRSF11B-OE-CM groups, respectively