Fig. 6

UCHL3 alleviates DFU by promoting the expression of FOXM1. (A) Immunohistochemical detection of FOXM1 expression in rat wound tissue. (B) Wound healing rate assessment in rats. (C) Blood glucose level monitoring in rats. (D) HE staining analysis of wound histology and morphology. (E) Immunohistochemical detection of CD31 (angiogenesis marker) in wound tissue. (F) Immunohistochemical detection of eNOS (endothelial function marker) in wound tissue. (G) WB analysis of angiogenesis-related proteins (VEGF and TSP-1) in wound tissues. n = 6. The magnification of images A, D,E and F is 100 times (scale = 400 μm) and 200 times (scale = 200 μm) respectively. ** P < 0.01, *** P < 0.001. The detection between the two groups was analyzed using t-test. Two factor analysis of variance (ANOVA) will be used for three or more sets of data, and Tukey’s will be used for post hoc testing