Fig. 7

DAS inhibits autophagy markers and apoptosis-related factors through the PI3K/AKT/mTOR signaling pathway. A–D Western blot analysis of the protein levels of p-AKT, T-AKT, p-PI3K, T-PI3K, p-mTOR and T-mTOR and the quantification of associated proteins in the blots shown. E–H western blot and quantitative correlation analysis of Beclin-1, LC3 and p62 in chondrocytes. I–L Western blot was performed to quantitatively analyze the expression of Bax, Bcl-2 and cleaved caspase-3. The values represent the mean ± SD. #p < 0.05 versus the control group. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group